الفهرس 
Origin of liquid chromatographyOnly 14 pages are availabe for public view
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Abstract
High performance liquid chromatography with ultra violet detector was used for quantification of rapamycin, a cyclic compound recently discovered as an immunosuppressive agent used in transplantation to keep the transplanted organ and prevent its rejection.
Extraction of blood rapamycin included two steps; the first step was the protein precipitation by using zinc sulphate solution and acetone thus, to get ride of protein compounds present in the sample that might absorb a part of ultra violet beam and give undesirable peaks in the chromatogram and interfere with rapamycin peak. The sample was then centrifuged to separate the upper layer which containing rapamycin from the precipitate. The second step was extraction of rapamycin present in this layer by adding 1-chJorobutane and sodium hydroxide. Further centrifugation was performed to separate the pure organic layer which containing rapamycin over the aqueous layer. The extract was then introduced into the chromatographic column.