الفهرس 
Material and methodsOnly 14 pages are availabe for public view
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Abstract
I)ISCUSSION
SUMMARY AND CO.NCLUSION
The present study was conducted to study the effect of
. estradiol benzoate injection postnatally on the development of the
testis in albino rats. Also, this study tried to enlighten the possible
mechanisms by which estrogen can affect the process of
spermatogenesis.
In this study, 75 male rats at the lSI day of life were used.
These rates were divided into three groups:
Group A: 25 male rats were injected sUbcutaneouslywith a single
dose of estradiol benzoate (E2B) 0.5 mg/5 gm B.W(low
dose).
Group B: 25 male rats were injected subcutaneously with a single
dose ofE2B 1 mg/5gm B.W (high dose).
Group C: 25 male rats were injected SUbcutaneouslywith a single
dose of normal saline (control group).
5 rats of each groups were killed at postnatal days 10, 22, 33,
45 and 60. The abdomen was opened and locati•on of the testes lI\~~v{\ 50
. detected. Then the testes were removed and preserved in Bouin’ s
solution, then stained by (Hx & E) and examined by light
microscope,
DISCUSSION
It was found that the testes of estrogenized rats remained
intraabdominally (while normally the testes reach the scrotum at day
22 old rats) and the size of the testes were smaller than normal.
The seminiferous tubules showed dilated lumena and impairment
of spermatogenesis with no sperm production until adult age.
The rete testis showed dilatation and anastomosing of their
channels, and there is impairment of spermatogenesis.These changes
in spermatogenesis were present at all parts of the testis (caudal and
cranial parts) of those treated with high dose E2B. On the other
hand, those treated with low dose of E2B, the cranial part of the
testis showed permanent impairment of spermatogenesis typical to
those treated with high dose while the caudal part showed less
influence and seemed more or less normal i.e., no dilatation of the
lumen, no sloughs, no inflammatory cells and the series of cells were
present till primary spermatocytes only.No spermatids or sperms
were observed. This was due to their existence further from rete
testis.
As the testicular descent in rat occurred after birth normally
and the human testicular descent occurred before birth, the results. of
this study are typical to that of human pregnant female exposed to
estrogen during pregnancy ..
In conclusion, our study can support the idea that estrogen
exposure to pregnant mothers leads to abnormalities in the male feti
reproductive system in the form of cryptorchidism and impairment
of spermatogenesis. So we advise to avoid exposure of female to.
estrogen during pregnancy.
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-4\ 97 ibIo
It was found that the testes of estrogenized rats remained
intraabdominally (while normally the testes reach the scrotum at day
22 old rats) and the size of the testes were smaller than normal.
.
The seminiferous tubules showed dilated lum~Ina and impairment
of spermatogenesis with no sperm production until adult age.
The rete testis showed dilatation and anastomosing of their
channels, and there is iITlP~irment.?f spermatogenesis. These changes
in spermatogenesis were present at all parts of the testis (caudal and
cranial parts) of those treated with high dose E2B. On the other
hand, those treated with low dose of E2B, the cranial part of the
testis showed permanent impairment of spermatogenesis typical to
those treated with high dose while the caudal part showed less
influence and seemed more or less normal i.e., no dilatation of the
lumen, no sloughs, no inflammatory cells and the series of cells were
present till primary spermatocytes only.No spermatids or sperms
were observed. This was due to their existence further from rete
testis.
As the testicular descent in rat occurred after birth normally
and the human testicular descent occurred before birth, the results of
this study are typical to that of human pregnant female exposed to
estrogen during pregnancy .
.- ~I--n, . conclu.=s’.io%n.,- our study can support the idea that estro.gen
exposure to pregnant mothers leads to abnormalities in the male feti
reproductive system in the· form of cryptorchidism and impairment
of spermatogenesis. So we advise to avoid exposure of female to
estrogen during pregnancy. -