الفهرس 
Material and methodsOnly 14 pages are availabe for public view
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Abstract
The effect of praziquantel on schislosoma mansoni infected
mice was studied.The aim of the present work is to :-
* Investigate the influence of the threrapeutic dose of praziquantel on
some liver functions tests.
* To demonsterate whether changes in some of the liver functions
(due to schistosomea mansoni ) could be arrested, reversed or
increased by praziquantel.
* To investigate the role ofpraziquantel as a prophylactic drug.
For this study 100 mice were used and divided into (5) groups each
contain 20 mice.
Group I Included 20 mice and were used as ( normal control ).
Group II: Included 20 non infected mice praziquantel was given at one
single dose of 200 mglkg body weight orally (Drug control ).
Group III: Included 20 mice infected by (100) schistosoma cerecariae
for each mouse using partial immersion technique ( Lennox
and Beuding, 1972).
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Group IV : Included 20 mice infected by (100)s-chistosoma cercariae
for each mouse using partial imniersion technique and
immediatly one single oral dose of praziquantel was given
by a dose of (200) mg/kg body weight (prophylactic group).
Group V: included 20 mice infected by (100) schistosoma mansoni
cercariae for each mouse using partial immersion technique
and after 45 days of infection the animals were teated with
praziquantel by a one single oral dose of praziquantel was
given by a dose of (200) mglkg body weight (treated group).
Sampling
,Blood samples were taken for each as follow:
Each mouse was slightly anaesthetized by ether, then its body
was fixed on dissecting plate.
The left axilla was dissected, then wasserman tube was put
under the left axilla away from the far of the mouse.
Axillary plexus was cut to left all the blood coming from the
heart to get inside the tube.
Blood samples of group II (Drug control) were taken after 10
days from given the drug.
Blood samples of group (III) and group (IV) were taken after 45
days from infection.
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Blood samples of group (V) were taken after 55 days [ 45 days
for infection and 10 day for treatment]
The sera were separated and stored at - 20°C for assaye.
The sera separated were used for determination of [serum AST,
ALT, GGT, total proteins, albumin, total bilirubin, direct bilirubin, indirect
bilirubin, alkaline phosphatase and protein fractionation].
Our results demostrated that there were significant increase in [
serum ALT, ALP, A/G ratio and albumin] while [ alph-l globulin%, alpha-
2 globulin % and beta globulin%] were significant decreased.
Our results demostrated that [ serum AST, total proteins and
gamma globulin %] were non significant increased while [serum total
bilirubin and indirect bilirubin] were non significant decreased (Drug
control ).
Our results showed that [ serum AST, ALT, GGT, total
bilirubin, alkaline phosphatase, total proteins, beta globulin% and gamma
globulin %] were significant increased while [ serum albumin, A/G ratio,
albumin% and alpha-l globulin%] were significant decrease.
Our results demonstrated that [ serum dirct bilirubin and alpha-2
globulin %] were non significant increased (Infected group).
Our results demonstrated that [ serum AST, ALT, GGT, alkaline
phosphatase total proteins, album, in, beta globulin% and gamma
globulin%] were significant increase•while [ serum total bilirubin, indirect
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bilirubin, albumin%, alpha-l globulin% and alpha-2 globulin%] were
significantlydecreased.
Our results demonstrated that serum direct bilirubin was non
significantly increased while serum total bilirubin was non significantly
decreased in (group V) (treated group) when treated group was compared
with control group.
/’
Our results showed that [ serum total proteins, albumin,A1G
t·)
ratio, albumin % and gammoglobulin%]were significantincreased while
oJ serum AST, ALT, GGT, total bilirubin, direct bilirubin, indirect bilirubin,
alkaline phosphatase, alpha-I globulin%, alpha-2 globulin% and beta
globulin%] were significantly decreased when treated group (group V)
was compared with infected group (group III).
Our results showed that [serum AST, ALT, GGT, alkaline
phosphatase, total proteins ablumin, and alpha-2 globulin%] were
significantlyincreased while beta globulin% was significantly decreased.
Our results showed that [ serum total bilirubin, indirect bilirubin,
alpha-l globulin % and gamma globulin %] were non significantly
increased while [ A1G ratio and albumin %] were non significantly
decreased when prophylactic group ( group IV) was compared with
normal control group.
Our results showed that [ serum albumin,A1G ratio, albumin%,
alpha -1 globulin% and alpha-2 globulin%] were significatly increased in
group IV ( prophylactic group) while [ serumAST, GGT, total proteins,
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total bilirubin, direct bilirubin, beta globulin % and gamma globulin %]
were significantly decreased.
Our results demonstrated that [ serum ALT and indirect
bilirubin] were non - significantly decreased when prophylaetic group
(group IV) was compared with infected group ( group III).
Our results showed that [serum AST, ALT, GGT, total proteins,
albumin, direct bilirubin, beta globulin % and gamma globulin% ] were
significantly increased while [ serum total bilirubin, indirect bilirubin, AlG
ratio, albumin%, alpha-l globulin %, and alpha-2globulin%] were
significanlty decreased. /’
Our results showed that serum alkaline phosphatase, was non ,,-
significanlty increased while serum ALT was non significantly increased
when treated group (group V) was compared with prophylactic group
(group IV).