الفهرس Only 14 pages are availabe for public view
 |  | from | 120 |  |  |
| | | from | 120 | | |
Abstract
Thermal injury is a serious medical, social, and economic problem. Almost every minute of the day, somewhere in the world a human being is becoming a victim of burns with their medical and social consequence which are often the cause of personal and family tragedies. Thermal injury destroys the skin barrier that normally prevent invasion by microorganism. So the bum wound is an excellent culture medium for the growth of a wide variety of bacteria and fungi due to the availability of nutrients, the moist environment and temperature. It is therefore not surprising that sepsis is a leading cause of morbidity and mortality in burned patients. Infection remains a major complication in burn wounds after the initial period of shock. More than 75% of their mortality has been attributed to infections, than to other causes. The present study aimed to: - To determine the most common bacterial and fungal agents encountered in the infection associated with burns.To determine the antibiogram of the isolated strains To study the E.M Pictures of resistant and sensitive strains of Ps. aeruginosa and S. aureus isolates. In this study 251 samples were collected from 153 burned patients in the burn Unit of Alexandria Main University Hospital (during the period from December 2005 to May 2006.) A questionnaire sheet was filled for every patient including all relevant information. These samples were taken from the patients. on admission and follow up swabs after one week were taken from. each patient an early morning before bathing of the patient or change of dressing, and on the appearance of any signs and symptoms of burn infection. All samples were subjected to: Dipping in Stuart’s transport medium and culture was on the following media: Blood, MacConkey agar, and Sabouraud’s Dextrose Agar media with and without cyclohexarriide and chloroamphenicol and incubated for 18-48 hat 37Co aerobically, for the other• tow media and at 30Cofor up to six weeks for the latter. Any isolated organism were identified by standard microbiological techniques .All isolates were tested for their antibiotic susceptibility by disc diffusion method described by Kirby-Bauer In case of samples examined by E.M.:one or two drops of broth suspension contained approximately 1-2xl06 of each of S.aureus (resistant and sensitive) and Ps. aeruginosa(resistant and sensitive). Were incubated at 37Co for 1 h then smears were done from each suspension, fixed in phosphate buffer glutaraldehyde overnight then washed repeatedly in distilled water followed by dehydration, in an ascending series of alcohol then coated with gold palladium. The specimen will be . examined by a JEOL. JSM-5300. Operated at 25kv.