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Abstract
A total of seven Bacillus thuringiensis (B.t.) isolates were obtained from thirty-four bacterial collections from different Egyptian soils. Two of these seven isolates were found to be effective against the 2nd instars of Spodoptera littoralis larvae. These isolates were named as B.t. Mo-I and K-II.
Cry I gene of these strains, which is responsible for insecticidal protein, was identified by polymerase chain reaction (PCR) technique. The morphology of sporangia and parasporal inclusions was differentiated by phase contrast and electron microscope, respectively. It was found that B.t.Mo-I produces bi-pyramidal inclusions, whereas K-II produces cubical inclusions.