الفهرس 
AcknowledgementOnly 14 pages are availabe for public view
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Abstract
The duodenal microflora is of paramount importance in relation to biliary tract and gast~oduodenal diseases, the~efore the aim of this work was, to study the normal duodenal flora, and its changes in cases of biliary tract and ’gastroduodenal diseases. Also to compare betwe~ the duodenal and bilia~y microflora in biliary tract diseases. This study was carried out on fifty five patients of both sexes and different ages, who were admitted to the surgi cal units of both, Alexandria University hospital and Karmouz Health Insurance hospital. The patients were divided into tt~ee groups: . Twenty patients were operated upon for biliary tract~iseases. Group A: Group B: Fifteen patients were operated upon for gastroduodenal diseases. Group C: Twenty patients as control group. Samples were taken from the duodenal contents of the three groups, in addition to samples from the bile of ~ group A. Samples were taken by aspiration using sterile plastic syringe. Samples were transported to the labora tory as quickely as possible on transport media. In the Laboratory, samples were inoculated on the following media: Two blood agar plates. MacConkey agar ~late. . Tomato juice agar plate. . All the plates were incubated aerobically at 37°C for 24 hours, while one blood agar plate and the Tomato juice agar plate were incubated anaerobically for 48 hours at 37°0. All the inoculated plates were examined and the microorganisms were identified by colonial characteristics, Gram-stain and. biochemical tests. IJ . In this study it was found that: 1) 60 of patients with biliary tract diseases were femaloes. While -;6.6 of patients with gastroduodenal diseases were males. 2) The duodenum yielded positive cultures in 50 of cases with biliary tract diseases, and in 46.6 of cases with gastroduodenal diseases, while 40 of the patients with bilharzial hepatic fibrosis (control group) showed positive duodenal culture. 3) In cases of biliary tract disease, it was found that, bile was infected in 25. 4) All the organi~ms, isolated from patients of the control group in absence of biliary tract and gastroduod enal diseases or intestinal obstruction, were Gram . ., posi ti ve organisms. 5) Gram negative organisms were isolated from 23.5 of cases wi~ biliary tract diseases and from 22.2 of cases with gastroduodenal diseases, while they were isolated from 42.9 in cases of intestinal obatruc tion.