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Abstract
The present investigation aims to study the toxicological, biologicl, biochemical and genetic effects of fungal (Abamectin) and pyrethroid (Esfenvalerate) insecticides on the spiny boloworm earias insulana (Boisd.). the obtained results revealed that, Abamectin was more effective against young eggs, larvae and adults, while Esfenvalerate was more potent against the pupae. The yound larvae were the most susceptible stage and the pupae were the most resistant stage to the effect of the two tested insecticides. Abamectin produced higher deleterious effects on the development of the spiny bollworm compared with that produced by Esfenvalerate. The two tested insecticides caused a slight depletion of total protein content in the haemdymph, at body and gut tissues of treated insects. The treatment with the two insecticides produced some differences in simple protein, lipoprotein, glycoprotein and SDS protein patterns (PAGE) in the three tissues of treated insects that the check. The analysis of isozyme esterases patterns was carried out by using four substrates and four inhibitors and revealed that the two tested insecticides caused the increasing of arylesterase and carboxyesterase activities and the inhibition of cholinesterase activities in the three larval tissues. The restriction Endo-nuclease assay and Random Amplified Polymorphic DNA (RAPD-PCR) analysis revealed some genetic differences between the untreated and treated larvae. Statistical analysis of obtained data revealed that the highest difference in DNA sequence was detected between the untreated and Abamectin treated larvae.