الفهرس 
IntroductionOnly 14 pages are availabe for public view
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Abstract
””””’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’!!!!!!!!!!!!!!! Summary
SUMMARY
1. Ten drinking water samples; six tap water and four bottled water,
were collection from different localities in Sharqia Governorate.
2. Physical and chemical analysis of the ten water samples revealed high
concentrations ofPb+2 & Fe+3ranging between 0.0204 - 0.0671 mg/l,
3. Bacteria naturally inhabiting water samples were isolated usmg
membrane filter technique.
4. The nine most frequent strains out of 127 were encoded I, II, III, IV,
V, VI, VII, VIII and IX.
5. The effect of increasing concentrations of Pb +2& Fe+3on growths of
nine tested strains revealed varying responses. Strains I, III, IV and
VIII survived high concentrations of Pb+2and Fe +3;(400& 500 ppm);
(450& 500 ppm); (500& 500 ppm) and (200& 500 ppm) respectively.
6. Examination the capability of the nine strains for Pb +2& Fe +3uptakes
revealed that strains I, III, IV and VIII were also the most efficient
isolates.
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7. The four most efficient and resistant bacterial isolates were identified
as Corynebacterium jeikeium, Pseudomonas putida biotype A,
Acinetobacter calcoaceticus and Acidovorax delafeldii.
8. Examining the efficiency of the four tested isolates for Pb+
2
& Fe+3
under static and shaking conditions revealed the inhibitory effect of
shaking.
9. Maximum biosorption of Pb+2 & Fe+3 by the four tested strains was
achieved at 100 ppm concentration.
IO.Studying the effect of different incubation temperatures revealed that
250C was optimum temperature except for Pb +2 uptake by
Acinetobacter calcoaceticus at 35°C.
l1.Removal of Pb+2 & Fe+3 from broth media was influenced by pH
values. Maximum removal capacities were obtained at pH 5 while pH
9 was suppressive value.
l2.Studying the effect f increasing concentrations of Cu+2 on growth of
the four selected strains showed that 50 ppm caused an increase in
CFUO/Oof Corynebacterium jeikeium, Pseudomonas putida biotype A,
Acinetobacter calcoaceticus. Meanwhile, Acidovorax delafeldii was
the most sensitive strain.
l3.The capability of four tested strains to uptake Cu+2 from their
solutions varied where they could be arranged descending in the
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’””””””””’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’’!!!!!!!!!! Summary
following order: Acinetobacter calcoaceticus, Corynebacterium
jeikeium, Pseudomonas putida biotype A and Acidovorax delafeldii.
14.Addition of Cu+2 ions caused significant decrease in Pb+2 & Fe+3
biosorption processmg revealing its antagonistic effect and
competition between different cations.
15.Using drinking water sample as metal- cell contact medium resulted in
decrease in biosorption of Pb+2& Fe+3compared to that in case of
nutrient broth medium. Yet the four strains accumulated promising
amounts of Pb+2 & Fe+3 (25.2& 17.1%); (34.6& 14.7%); (30.7&
19.5%) and (28.3& 12%) respectively.
16.Plasmid profile of the four selected strains showed that Acinetobacter
calcoaceticus contained plasmid of size 23.13bp while the other three
strains were plasmidless.
17.Ultrastructure study using transmission electron microscope revealed
that Pb+2 was adsorbed and accumulated by Acinetobacter
calcoaceticus on cell surface. Meanwhile, Fe+3 was absorbed and
accumulated intracellularly.
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