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Abstract
New methods for determination of microbial adherence
and colonization to biomaterial surface pre and post- irradiation treatment in cancer patients.
The present study aimed to prove that adherence and colonization of biomaterials’ surface by pathogenic microorganisms and biofilm formation as a virulent factor may pose a public health problem for persons requiring the use of biomaterials such as urinary catheters, and to study the relation between biofilm formation and antimicrobial-drug resistance. Also, to study the effect of radiotherapy of bladder cancer patients on the microorganisms, their antibiotic sensitivity, adherence and extracellular polysaccharide slime production as well as the plasmid profile and algD gene which plays an important role in the adherence process of some selected strains. The results could be summarized as follows:
1- The study was carried out on a total of 100 urinary catheters, and 100 urine samples from the attached drainage bags from 100 bladder cancer patients undergoing external pelvic radiotherapy by Theratron 780E Cobalt 60 unit and attending out-patients and in-patients clinics at the National cancer Institute, Cairo University. Samples were taken prior to the start of the radiotherapy.
2- Isolation and identification of pathogenic bacteria and yeasts were carried out. The results included 98 (98%) positive infected cases. The total number of pathogenic bacteria and yeasts was 146 isolates. Out of them 123 (84.25%) bacterial isolates and 23 (15.75%) yeast isolates.
3- There were 23 species of pathogenic bacteria isolated belonging to 11 genera and 13 species of pathogenic yeasts belonging to 8 genera.