الفهرس 
introductionOnly 14 pages are availabe for public view
 |  | from | 332 |  |  |
| | | from | 332 | | |
Abstract
The present study was Performed to assess the chemopreventive potential of Nigella sativa seeds and/or Allium sativum powder as well as Nigella sativa fixed oil on colon cancer induced growing male Sprague Dawley rats.Colon cancer was induced by weekly subcutaneous injection of 1, 2 dimethylhydrazine dihydrochloride (DMH) at a dose of 20 mg/kg body weight for 20 weeks. The colon cancer¬ induced rats were classified into seven groups. One group received the basal diet and served as colon cancer positive control group. The other six groups received the experimental supplemented with Nigella sativa seeds (2.5% or .5%), Allim sativum powder (2.5% or 5%), Nigella sativa fixed oil (2%) or a mixed dose of the two plants (5% of each). Another, group of healthy rats was kept on the basal diet, injected with saline solution and represented the negative control group.
Rats were fed the dietary regimens for 4 weeks. before DlYM. injection, and throughout the’ study period, Feed consumption and body weight change were recorded at weekly Relative weights of some organs were recorded at time of sacrificing. The measured parameters were: colonic and hepatic malondialdehyde . (MDA) levels, reduced glutathione content (GSH) and the activities of colonic and hepatic catalase, erythrocyte Cu/Zn superoxide dismutase (Cu/Zn SOD) glutathione reductase (GR) and blood glutathione peroxidase (GPX) as well as plasma selenium concentration ’(Se), plasma a tocopherol Content and plasma nitric oxide levels (NO). Histopathological examination of colon and liver was also performed.
Proximate analyses of Nigella sativa seeds and Allium physiochemical sativum powder fatty acids profile and physiochemical properties of Nigella sative fixed oil as well as microbiological properties of examination of these materials were also carried out.