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Abstract
The usage of minimally invasive procedures and attention to patient comfort, especially for dental treatment of children, have led to the development of chemomechanical methods of caries removal.
The purpose of this controlled clinical trial was to evaluate the effect of the chemomechanical method of caries removal (Carisolv) on the residual bacteria in the occlusal cavities of primary molars in comparison to conventional hand excavation.
Thirty children, aged 5-7 years with a mean age of 5.4 ± 0.83 years were selected for this study. The selected children had two similar bilateral carious primary molars, requiring Class I cavity preparation. A total of 60 teeth were included in the study. Children were recruited from those attending the out-patient clinic, Pediatric Department, Faculty of Dentistry, Alexandria University. The teeth were randomly divided into two equal groups according to the method of caries removal. Group I included 30 teeth in which caries was removed using the chemo-mechanical method (Carisolv), whereas group II included 30 teeth in which caries was removed using the mechanical method (conventional hand excavators).
Samples from carious dentin were taken with a sterile sharp excavator, then all softened dentin was removed with either method and a second sample was taken when the cavities were clinically judged caries-free. Then, all molars were filled with composite resins. All samples were transported to the laboratory and were serially diluted and cultured anaerobically on different agar plates; blood agar, Mtis salivarius agar and Rogosa agar plates. Colony forming units were determined for total bacterial counts, mutans streptococci and lactobacilli counts.